In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells
Joint Authors
Sasikumar, Raju
Das, Arub Jyoti
Deka, Sankar Chandra
Source
Saudi Journal of Biological Sciences
Issue
Vol. 28, Issue 9 (30 Sep. 2021), pp.5338-5348, 11 p.
Publisher
Publication Date
2021-09-30
Country of Publication
Saudi Arabia
No. of Pages
11
Main Subjects
Abstract EN
Cyanidin-3-glucoside (C3Ghv) compounds were purified and isolated from the anthocyanins extract of Haematocarpus validus.
C3Ghv were studied for antioxidant and cytoprotective properties on pancreatic β-cells of rat insulinoma cells (RINm5F) against the oxidative stress induced by streptozotocin (STZ).
The exposure of RINm5F cells to C3Ghv at concentration of 100 and 200 μg/mL for 24 h reduced 10% and 23% cell viability, respectively, as compared to control cells.
The pre-treatment of RINm5F cells with C3Ghv (50 µg/mL) increased the cell viability by 29% as compared to control, on being treated with STZ (10 mM) for 24 h.
The pre-treatment of RINm5F cells with C3Ghv (50 µg/mL) for 24 h followed by exposure to STZ (10 mM) for 1 h decreased the generation of reactive oxygen species (ROS) by 57%, generation of nitric oxide by 22.8%, generation of malondialdehyde (MDA) by 32%, the production of p-ERK ½ by 83%, p-JNK by 82.6%, p-MEK by 57%, and p-p38 MAPK by 64%.
The C3Ghv treatment also decreased the ratio of apoptotic proteins Bax to Bcl-2 by 61%, and improved the M2 phase of cell cycle by 75% as compared to STZ treated cells.
The overall results suggest that C3Ghv protects pancreatic β-cells against oxidative stress-induced apoptosis, thereby implicating the significant role of C3Ghv as an antidiabetic agent.
American Psychological Association (APA)
Sasikumar, Raju& Das, Arub Jyoti& Deka, Sankar Chandra. 2021. In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells. Saudi Journal of Biological Sciences،Vol. 28, no. 9, pp.5338-5348.
https://search.emarefa.net/detail/BIM-1411387
Modern Language Association (MLA)
Sasikumar, Raju…[et al.]. In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells. Saudi Journal of Biological Sciences Vol. 28, no. 9 (2021), pp.5338-5348.
https://search.emarefa.net/detail/BIM-1411387
American Medical Association (AMA)
Sasikumar, Raju& Das, Arub Jyoti& Deka, Sankar Chandra. In vitro cytoprotective activity of cyanidin 3-glucoside extracts from Haematocarpus validus pomace on streptozotocin induced oxidative damage in pancreatic β-cells. Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 9, pp.5338-5348.
https://search.emarefa.net/detail/BIM-1411387
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 5347-5348
Record ID
BIM-1411387