كشف الأنواع الفطرية المفرزة لسموم الأفلاتوكسينات باستعمال التقانات الحيوية الجزيئية

Abstract EN

Mycotoxins cause economic losses and health problems for humans and animals, like immunity weakness, mutations, cancer and death if consumed at high quantities.

This research aimed to detect the fungus strains which secrete the aflatoxins and to define aflatoxins types using official methods, and to discriminate the aflatoxinogenic and nonaflatoxinogenic strains by PCR technique to detect the existence of two genes aflR and omt-1.

Eleven fungal strains as aflatoxigenic species belonging to A.

flavus and A.

parasiticus were defined from 20 strains.

The percentage of aflatoxigenic strains of A.

flavus was 80%, none of them produced aflatoxins G1 and G2.

The species A.

oryzae, A.

Niger, penicillium verrucosum and Fusarium verticillioides did not produce any type of aflatoxins.

Results showed that aflatoxin B1 was the most secreted, and statistical analysis showed significant differences between strains in secreting specific aflatoxin.

Strain sy 8 of A.

Flavus secreted the highest concentration of aflatoxin B1 (1782.9 ppb).

This research concluded that PCR technique is fast, sensitive, less cost and provide more precaution, and it can be used for primary detection of aflatoxigenic fungi.