تأثير خلاصة أوراق الزيتون السوري في تثبيط فاعلية الأميلاز ألفا مخبريا

Abstract EN

Some compounds of the Syrian olive leaves extract have been identified by high-performance liquid chromatography coupled with a mass spectrometry.

As expected oleuropein was found to be the most abundant compound in these extracts.

The study also examined the effectiveness of ethanol and aqueous extracts of Syrian olive leaves in inhibiting the α-amylase enzyme in order to investigate the ability of these extracts to reduce blood sugar in vitro.

Inhibition percentage reached 77.74% at 6.14 mg/ml concentration of olive leaves extracts.

The amylase inhibition pattern was also tested, the max velocity Vmax and Micheles - Menten constant Km were calculated from the Lineweaver-Burk equation.

The result showed that inhibition is uncompetitive type.