Comparison of two genomic DNA extraction protocols from single dray seed of barley crop
Joint Authors
Shaban, Ahmad
Abu Sunaynah, Muhammad
al-Hajjaji, Khalid
al-Abid, Kairy
Abu Ghaniyah, al-Mundhir
Source
Scientific Journal for the Faculty of Science-Sirte University
Issue
Vol. 2, Issue 1 (30 Apr. 2022), pp.126-131, 6 p.
Publisher
Sirte University Faculty of Science
Publication Date
2022-04-30
Country of Publication
Libya
No. of Pages
6
Main Subjects
Abstract EN
Modern plant breeding studies are largely based on plant genetic engineering programs.
Extraction of DNA with high quality plays as a key factor in most of plant genetic Studies, therefore two different DNA extraction protocols based on CTAB buffer and SDS buffer were tested for the purpose of selecting the best DNA extraction protocol from dry seeds of nine variety of barely plant .
Barley seeds were taken from Libyan seeds gene bank .After Barely samples were prepared DNA was extracted directly using CTAB and SDS solutions .The quality of extracted DNA was assessed by spectrophotometric measurements and gel electrophoresis system.
The results of this study showed that purity of extracted DNA by CTAB method was clearly batter compared with SDS method.
CTAB method seemed to be more effective for extracting DNA from barely dry seeds.
High quality DNA obtained through use of CTAB method, while CTAB had overall better A260/A280 ratio (1.736-1.932).
SDS method seemed to be not suitable for DNA extraction from dry seeds of barely crop.
American Psychological Association (APA)
Shaban, Ahmad& Abu Sunaynah, Muhammad& al-Hajjaji, Khalid& al-Abid, Kairy& Abu Ghaniyah, al-Mundhir. 2022. Comparison of two genomic DNA extraction protocols from single dray seed of barley crop. Scientific Journal for the Faculty of Science-Sirte University،Vol. 2, no. 1, pp.126-131.
https://search.emarefa.net/detail/BIM-1440055
Modern Language Association (MLA)
Shaban, Ahmad…[et al.]. Comparison of two genomic DNA extraction protocols from single dray seed of barley crop. Scientific Journal for the Faculty of Science-Sirte University Vol. 2, no. 1 (Apr. 2022), pp.126-131.
https://search.emarefa.net/detail/BIM-1440055
American Medical Association (AMA)
Shaban, Ahmad& Abu Sunaynah, Muhammad& al-Hajjaji, Khalid& al-Abid, Kairy& Abu Ghaniyah, al-Mundhir. Comparison of two genomic DNA extraction protocols from single dray seed of barley crop. Scientific Journal for the Faculty of Science-Sirte University. 2022. Vol. 2, no. 1, pp.126-131.
https://search.emarefa.net/detail/BIM-1440055
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 131
Record ID
BIM-1440055