Design and construction of a localized surface plasmon resonance-based gold nanobiosensor for rapid detection of brucellosis
Joint Authors
Nimah Allahi, Muhammad Hadi
Asadikaram, Gholamreza
Savardashtaki, Amir
Vakili, Sina
Mahani, Masud Torkzadeh-
Behroozikhah, Ali Mohammad
Source
Issue
Vol. 48, Issue 3 (31 Jul. 2021), pp.1-9, 9 p.
Publisher
Kuwait University Academic Publication Council
Publication Date
2021-07-31
Country of Publication
Kuwait
No. of Pages
9
Main Subjects
Abstract EN
In this study, a localized surface plasmon resonance (LSPR) nanobiosensor was designed to quantify anti-Brucella antibodies in the human sera.
Smooth Lipopolysaccharide (LPS) was extracted from Brucella melitensis via a modified hot phenol water method and fixed on the surface of the gold nanoparticles by covalent interactions with the functionalized nanoparticles.
To obtain the best performance from the designed probe, the ratio of LPS to gold nanoparticle was optimized.
Dynamic light scattering was used for the characterization of the probe.
The reduction of the LSPR peak at 600nm was used to quantify the amount of captured anti-Brucella antibody.
Finally, satisfactory results were obtained when the nanobiosensor was used to analyze the control and patient sera for the presence of anti-Brucella antibodies.
American Psychological Association (APA)
Vakili, Sina& Asadikaram, Gholamreza& Mahani, Masud Torkzadeh-& Behroozikhah, Ali Mohammad& Nimah Allahi, Muhammad Hadi& Savardashtaki, Amir. 2021. Design and construction of a localized surface plasmon resonance-based gold nanobiosensor for rapid detection of brucellosis. Kuwait Journal of Science،Vol. 48, no. 3, pp.1-9.
https://search.emarefa.net/detail/BIM-1503384
Modern Language Association (MLA)
Vakili, Sina…[et al.]. Design and construction of a localized surface plasmon resonance-based gold nanobiosensor for rapid detection of brucellosis. Kuwait Journal of Science Vol. 48, no. 3 (Jul. 2021), pp.1-9.
https://search.emarefa.net/detail/BIM-1503384
American Medical Association (AMA)
Vakili, Sina& Asadikaram, Gholamreza& Mahani, Masud Torkzadeh-& Behroozikhah, Ali Mohammad& Nimah Allahi, Muhammad Hadi& Savardashtaki, Amir. Design and construction of a localized surface plasmon resonance-based gold nanobiosensor for rapid detection of brucellosis. Kuwait Journal of Science. 2021. Vol. 48, no. 3, pp.1-9.
https://search.emarefa.net/detail/BIM-1503384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-9
Record ID
BIM-1503384