تأثير كلوريد الصوديوم (NaCl)‎ على بعض الفطريات المعزولة من التربة

Abstract EN

This study has been conducted to isolate and identify some fungi from cultivated soil from Assekt area in the city of Misurata.

Some of it was isolated by the method of loosening.

It was grown and purified in P.D.A medium.

Then, it was diagnosed on the basis of its culture and microscopic characteristics.

The isolated fungi were represented by 6 fungal genera : (Penicillium sp Aspergillus Niger Aspergillus flavus Alternaria alternata_ Fusarium oxysporum_Rhizoctonia sp).

Then they were grown in a culture medium and sodium chloride salt (NaCl) was added to it at different concentrations (10%, 20%, 30%), to measure its effect on fungal growth rates.

The study results have shown a clear effect and change in fungal growth rates, as the growth rate has been measured in two ways.

(Measuring the diameter of the colony, and measuring the dry weight of the fungus).

It has been noted that the fungus F.

oxysporum recorded the highest growth rate at the concentration of 10% compared to the rest of the fungi at all concentrations, with a growth rate of (9 (cm) when measuring the diameter of the colony, while when measuring the dry weight it was recorded A.

flavus fungus reached the highest growth rate with (0.168g) in a 20% concentration, while A.

Niger mushroom recorded the lowest growth rate in a 20% concentration when measuring the dry weight with a growth rate of (0.031(g) compared to other fungi in different concentrations.

Penicillium fungus sp also recorded the lowest growth rate when measuring the diameter of the colonies at a growth rate of (3.5g) compared to the rest of the fungi at different concentrations.

The results of the study revealed that the growth of fungi isolated from agricultural soils differed according to the type of fungi and the concentrations of (NaCl) added to the test medium, and that their growth rates differed even in one concentration.

This was proved when comparing the diameters of the growing colonies and their dry weight in the presence of different salt concentrations.

It was noted that in the media containing concentrations of 10% and 30%, it showed the best growth of the fungi under test with an average growth of (6cm), followed by a concentration of 20% with an average growth of (5cm) while measuring the diameters of the colonies, while measuring the dry weight showed that the best growth was in the concentration of 10%, 20% and then 30% with an average growth of (0.119g, 0.107g, 0.101g), respectively.