دراسة العلاقة بين لون صبغات الشعر و تأثيراتها في الزجاج على الخط الخلوي HEK293T

Abstract EN

Background & Aim : Hair dyes are commonly cosmetic preparations used to color hair.

Despite the debates about their safety, there are no data confirming their hazards yet.

Six permanent hair dyes with the colors : medium brown, dark brown, black, dark red, smoky blond and beige blond as described on the commercial container were studied.

The study evaluated the toxicity of the absorbed portion of hair dyes on HEK 293T cell line in vitro.

Materials & Method : The solutions used in treatments were prepared by Franz cell, through applying the hair dyes of cell membrane for 45 mins and then the receptor fluids were preserved.

The cell line was treated with four volumes of dyes solutions that were prior ordered.

Then the treated cells and the control were incubated at 37°C in 5% CO2 incubator for 24 hours, then the cell viability was tested by MTT assay.

Results : The results showed a statistical increase in cell number when adding the dyes solutions of blond color in two bands, red and black colors.

Whereas no statistical significant increasing caused by dark brown color was found.

Only the brown color caused a decrease in the number of cells.

Conclusion : Consequently, the studied dyes of blond color in the two bands, red and black color were inducer to cell growth in vitro.

On the contrary, the medium brown dye was an inhibitor.

Dark brown dye was considered as the safest, with regard to the composition of the studied dyes that were determined by the company.