تأثير نوع الوسط الغذائي ومنظمات النمو على الاكثار الدقيق لنبات خف الجمل . Bauhinia purpurea

Abstract EN

This study was conducted during the years 2016 - 2017 in Plant Tissue Culture Laboratory at the Agriculture Research Center in Sidi El-Massri Station, Tripoli.

The objective of this study was to establish a microproagtion protocol for Bauhinia purpurea L.

Shoots were taken from in vitro grown seedlings after 45 days of culture and prepared into single nodes of 1.0cm length carrying a single bud, cultured on Murashige and Skoog medium (MS) or Loyd and McCown medium (WPM) containing different concentrations of Benzyl adenine (BA) alone or in combination with 1 mgL" Naphthalene acetic acid (NAA) for multiplication.

For rooting, shoots which were produced from single nodes, were cultured on 14, ½ or full strength MS basal salts media supplemented with different concentrations of Indole butyric acid (IBA) or NAA with or without 0.3% activated charcoal.

The results showed that MS Medium containing 1.5 mgL BA alone or in combination with 1 mg L of NAA gave the best results based on shoot number and shoot length (4.5 and 7.3 cm) and highest leaf number.

Rooting results showed that, shoots which were cultured on ½ MS medium supplemented with 5 mgL IBA containing 0.3% activated charcoal had the highest rooting percentage (88.8 %), number of roots (6.6) and length of root (3.6cm).

Plantlets were successfully acclimatized in a sterile growing mixture composed of soil with peat moss at 1 : 1 (v/v) with a survival rate 75% after 6 weeks.