تأثير المعاملات المختلفة في إنتاج مادة إكثار نباتية من صنف الكرمة الحلواني خالية من فيروس التفاف أوراق الكرمة مختبريا

Abstract EN

This study was carried out during the period 2020-2021, to produce virus-free propagation material from Halwani cultivar of grape infected with Grapevine leaf roll-associated virus 3 (GLRaV-3), by using the meristem tip culture technique together with thermal and chemical treatments.

The meristem tip of 500 micrometers was excised from infected plants and placed on 1/2 MS medium, which contains 0.5 mg/1 BAP and 30 g/l sucrose.

Virus presence or absence was confirmed by the ELISA test (DAS-ELISA).

Results showed that the highest Percentage of virus free plantlets (87.67%) was obtained from the combined treatment, whereas the thermal treatment resulted in the lowest percentage of virus-free plants (56.25%).

The results obtained also showed the significant superiority of using meristem-tip culture compared with all other treatments in terms of percent growing plants, which reached 98.13%.

This study indicated that there were negative effects of all treatments on plant elongation, especially the thermal treatment, which gave the least plant growth elongation (0.33 cm).