تعيين خصائص ثبات الغلوبولينات المناعية lgGs لجمل ممنع تجاه سم العقرب وتنقيتها وكشفها

Abstract EN

Immunoglobulins are at the heart of many biological applications, including the treatment of diseases.

They have been produced and used to investigate many antigens and pathogens that are studied globally and locally.

Currently, using the methods of molecular biology and genetic engineering, it is sought to invest a specific type of antibodies that is characteristic of camels to produce effective sera against pathogens or to be used as toxins antidote of dangerous creatures, especially scorpion venom.

In this work, antibody classes were isolated from immunized camel serum by ammonium sulfate precipitation and fast protein liquid chromatography (FPLC).

In order to get rid of ammonium sulfate residues, desalting column was used.

Then IgGs were purified using protein A column and IgGs types were separated using a Superdex column.

Purified camel IgGs were tested and characterized using ELISA, which in turn proved the efficacy and quality of the isolated IgGs types against scorpion venom.

These findings constitute an important step towards conducting pharmaceutical formulation studies for these promising antibodies.