التنوع الوراثي في الشعير باستخدام المؤشرات الجزيئية

Abstract EN

A core collection representing a barley collection covering the WANA region, was chosen to represent the genetic spectrum of the whole collection.

The DNA samples of 315 accessions were assayed for RAPD-PCR using three primers.

The results with the three primers allowed to regroup the 315 accessions into (97) different patterns.

The different patterns represent the genetic diversity detected between the accessions analyzed and its distribution across the countries.

Two kinds of patterns were detected: a frequent pattern which is present in several countries and a specific pattern which is present in one country only .

The frequency of each pattern across countries indicates the distribution of the diversity between countries.

Our results showed that the RAPD assay could be a useful tool for assessing genetic diversity.

The level of genetic diversity in the core collection tested is large.

Although we used only three primers, we were able lo distinguish between the majority of accessions and to regroup them into 97 clusters.