The antimicrobial effect of alcoholic ex¬tract of olive leaves as a root canal ir-rigant

Abstract EN

-Aims: The study aims to evaluate the antimicrobial effect of different concentrations of alcoholic extract of Olive Leaves solution (0.1%, 0.2%, 0.4%, 0.6%, 0.8%) in an in vitro.It also to determines the time required for the most effective concentration of Olive Leaves to start the effect in selected microorganisms, and to evaluate the antimicrobial effect of most effective concentrations of alcoholic extract of Olive Leaves solution in an in vivo study as a root canal irrigant.

Materials and Methods: The antimicrobial effect of Olive Leaves (0.1%, 0.2%, 0.4%, 0.6%, 0.8%), 2.5% sodium hypochlorite, and normal saline were determined in vitro by using broth microdilution method.

The direct exposure test was used to evaluate the time required for 0.8% of Olive Leaves to start their antimicrobial effect on the selected microorganisms.

In an in vivo study, 36 uniradicular teeth with necrotic pulps were chosen.

The patients were divided randomly into three groups, 12 patients for each group as in the following: Group I: alcoholic extract of Olive Leaves at 0.8%.

Group II: sodium hypochlorite solution at 2.5% as a positive control.

Group III: normal saline as a negative control.

Bacteriological samples were obtained from the canal at the beginning of the first appointment; at the beginning of the second appointment; at the end of the second appointment; at the beginning of the third appointment using sterile wet paper point.

Results: The Results showed that Olive Leaves at (0.2%, 0.4%, 0.6%, 0.8%), and sodium hypochlorite had a significant antimicrobial effect against aerobic and anaerobic bacteria recovered from teeth with necrotic pulps.

While the normal saline and 0.1% Olive Leaves had no significant antimicrobial effect.

As for Olive Leaves exract.

The best antimicrobial effect was noticed at 0.8%, which showed a significant difference from other concentrations of Olive Leaves,but no significant difference from that of sodium hypochlorite.

The results of this test showed that 2.5% sodium hypochlorite and 0.8% Olive Leaves had immediate effect on all selected microorganisms.

The results revealed that 0.8% alcoholic extract of Olive Leaves solution had a significant antimicrobial effect when utilized clinically as an endodontic irrigant, which was not significantly differ from sodium hypochlorite but significantly different from normal saline.

Conclusions: Olive Leaves alcoholic extract solution at 0.8% was an effective antimicrobial agent when used as an irrigant in endodontic treatment of teeth with necrotic pulps.

Key Words: Antimicrobial Effect, Alcoholic Extract, Olive Leaves, Root Canal Irrigant.