استخدام المخلفات الزراعية السليلوزية لإنتاج إنزيم glucose isomeras الخارج خلوي بوساطة البكتريا المحبة للقاعدية

Abstract EN

Thirty-two alkalophilic bacterial isolates producing glucose isomerase enzyme were isolated from soil samples, using liquid medium at initial pH of 9 supported with (lOml/L) of xylose extract from straw (H2SO4 straw hydrolysate).

The ability of isolates were tested to produce extracellular Glucose isomerase enzyme in the liquid medium.

Results showed that the BC4 isolate gave high ability to produce Glucose isomerase enzyme 1.2 unit/ml, subsequently was identified and designated as Bacillus sp.

The optimum conditions (Temperature, time and pH) for extracellular Glucose isomerase production from Bacillus sp.

isolate were studied.

Results indicated that the isolate showed highest enzyme production (1.9 unit/ml) when grown in a liquid medium at initial pH of 10 and subsequent incubation at 35C for 72 hrs.