دراسة وراثية لبكتيريا Aeromonas المنتجة لأنزيمات البيتالاكتاميز الصنف C

Abstract EN

Five hundreds and seven samples were collected from different environmental and clinical sources .

Only, 17 isolates were belonging to the genus Aeronionas after doing morphological and biochemical tests .The ability of Aeromonas isolates to produce P-lactamase class C enzymes were tested, and the results showed that only two isolates ( A .

sobria and A.

eucrenophila ) produced this type of enzyme.The antibiotic scussptibility tests against 15 antibiotics were examined .

and it was found that the two isolates were resistant to penicillin, ampicillin, ampiclox, amoxicillin, lincomycin, cefoxitin and cefotaxime, while they were sensitive to ceftazidime.

Agarose gel electrophoresis showed that Aeromonas isolates have two small plamid bands .

Transformation experiments revealed that the small plasmid bands were capable to transform E.

coli MM294, an observation which indicates the ability of these plasmids to show their expression in more than one host.