Effects of different concentrations of melatonin on the time-course of nitrite-induced oxidation of hemoglobin : in vitro study

Other Title(s)

تأثير التراكيز المختلفة من الميلاتونين على مدة العلاج وأكسدة الهيموغلوبين التي تسببها النترات : دراسة مختبرية

Joint Authors

Numan, Intesar T.
Muhammad, Shayma Mundhir
Abd al-Wahhab, Ihab I.
Husayn, Sad Abd al-Rahman

Source

Iraqi Journal of Pharmaceutical Sciences

Issue

Vol. 18, Issue 1 (31 Mar. 2009), pp.78-81, 4 p.

Publisher

University of Baghdad College of Pharmacy

Publication Date

2009-03-31

Country of Publication

Iraq

No. of Pages

4

Main Subjects

Pharmacy, Health & Medical Sciences

Topics

Abstract AR

إن عملية أكسدة الهيمغلوبين و تحوله إلى ميتهيمغلوبين قد تمت دراستها بشكل واسع، و تهدف الدراسة الحالي إلى تقييم مقدر تراكيز من مادة الميلاتونين على منع أو تأخير حدوث عملية الأكسدة بمادة نايترايت الصوديوم.

تم الحصول على عينات دم من أشخاص أصحاء و تحضير محلول من الهيمغلوبين من الكريات المتحللة و حسب الطرق المعتمدة منن قبل الآخرين.

تم مزج محلول الهيمغلوبين مع تراكيز مختلفة من مادة الميلاتونين (9-10-1 ملغم / مل) لمدة 10 دقائق تم بعدها إضافة مللتر واحد من مادة نايترات الصوديوم كعامل مؤكسد.

تمت متابعة عملية التأكسد من خلال قياس مستوى الميتهيمغلوبين المتكون كل دقيق باستخدام مطياف الأشعة فوق البنفسجية.

أظهرت النتائج أن للميلاتونين القدرة على تأخير تأكسد الهيمغلوبين بصورة تعتمد على التركيز و فترة الخلط.

و يمكن الأستنتاج أن الميلاتونين بإمكانه حماي الهيمغلوبين من التأكسد بواسطة نايترات الصوديوم و بصورة تعتمد على التركيز و فترة المزج.

Abstract EN

Melatonin is a potent scavenger of reactive oxygen species or free radicals like superoxide and hydroxyl radicals.

The oxidation of hemoglobin to meet hemoglobin (meth-Hb) by oxidizing compounds has been widely studied.

The present work was designed to evaluate the ability of different concentrations of melatonin to inhibit nitrite–induced oxidation of hemoglobin.

Blood samples were obtained from apparently healthy individuals from which erythrocyte hem lysate was prepared.

Different concentrations of melatonin (10-9-1.0 mg / ml) were incubated for 10 min with the hem lysate, then to the resultant mixture 1 ml of sodium nitrite (final concentration 0.6 mM) was added, and the formation of meth-Hb was measured by monitoring absorbance of light at 631 nm each min for 30 min.

Control samples without melatonin were utilized for comparison.

Nitrite caused rapid oxidation of hemoglobin to meth-Hb in control samples; in the presence of melatonin, the oxidation process was delayed in a dose–dependent manner.

The effect of melatonin on the time course of nitrite-induced oxidation of Hb showed that melatonin has a protective effect initiated early after addition along with nitrite.

Melatonin also affects the time required for the formation of meth-Hb, the time required to convert 50 % of the available Hb to meth-Hb was 4 min in the absence of melatonin, and became 17, 22, 26, 30, 114, and 383 min with increasing melatonin concentrations (10-9, 10-6, 0.001, 0.01, 0.1, and 1.0 mg / ml respectively).

In conclusion, melatonin in a concentration and time dependent manner can protect Hb from oxidation by nitrite; melatonin delays the onset of autocatalytic stage and the protective effect extended over long period of time.

American Psychological Association (APA)

Husayn, Sad Abd al-Rahman& Muhammad, Shayma Mundhir& Numan, Intesar T.& Abd al-Wahhab, Ihab I.. 2009. Effects of different concentrations of melatonin on the time-course of nitrite-induced oxidation of hemoglobin : in vitro study. Iraqi Journal of Pharmaceutical Sciences،Vol. 18, no. 1, pp.78-81.
https://search.emarefa.net/detail/BIM-289029

Modern Language Association (MLA)

Husayn, Sad Abd al-Rahman…[et al.]. Effects of different concentrations of melatonin on the time-course of nitrite-induced oxidation of hemoglobin : in vitro study. Iraqi Journal of Pharmaceutical Sciences Vol. 18, no. 1 (Mar. 2009), pp.78-81.
https://search.emarefa.net/detail/BIM-289029

American Medical Association (AMA)

Husayn, Sad Abd al-Rahman& Muhammad, Shayma Mundhir& Numan, Intesar T.& Abd al-Wahhab, Ihab I.. Effects of different concentrations of melatonin on the time-course of nitrite-induced oxidation of hemoglobin : in vitro study. Iraqi Journal of Pharmaceutical Sciences. 2009. Vol. 18, no. 1, pp.78-81.
https://search.emarefa.net/detail/BIM-289029

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 81

Record ID

BIM-289029