إكثار نبات الشبو الشجيري cestrum nocturnum L خارج الجسم الحي (in vitro)‎

Abstract EN

Tissues culture technique was conducted to initiate and multiplicate different explants of cestrum nocturnum plant.

Rooting the shoots produced plants and transferred to greenhouse after acclimation.

During initiation stage, ability of different explants Apical shoots, single node cuttings, immature inflorescence bud, leaf blades, leaf petioles and internodes were tested for shoots proliferation when cultured on solid MS medium supplemented with (0, 1, 2, 3, 4) mg/l BA , after 4 weeks of culturing, best results where obtained from single node cuttings, apical shoot and immature inflorescence buds respectively, in medium supplemented with 2 mg/L BA, while the other explants were failed to produce shoots.

Depending on this result we tested the ability of single nods and apical shoots for multiplication during culture on medium supplemented with (0, 1.5, 2.0, 2.5) mg/L BA alone or with NAA at concentration (0.1, 0.3, 0.5) mg/L, after 8 weeks from culturing in the multiplication medium the best result was achieved when subculturing in the medium supplemented with 2.5 mg/L BA + 0.3 mg/L NAA which produce 6.7 shoots/single nodes and 4.0 shoots/apical shoot.

Shoots produced from multiplication stage were rooted at 90% when cultured on 1/2 MS salts medium supplemented with 0.5 mg/L IBA.

Acclimated plants grew well under greenhouse conditions.