Factors affecting the production of β-galactosidase in different strains of enterobacter aerogenes
Other Title(s)
العوامل المؤثرة في إنتاجية إنزيم بيتا-جالكتوسيديز في أنواع مختلفة من enterobacter aerogenes
Dissertant
al-Shurafa, Khalid Yusuf Ahmad
Thesis advisor
Comitee Members
Khulayfat, Khalid Muhammad
al-Shawabikah, Riyad
Kanan, Ghassan Jaddu
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2004
English Abstract
Growth conditions which were expressed as biomass or OD60o nm as well as the production of p-galactosidase enzyme were investigated in different strains of Enterobacter aerogenes.
Three Enterobacter strains were included in this studies and there are pUC8:16 ; A transformant carrying Vitreoscilla hemoglobin gene (vgb) and two control strains.
The first (pUC9) is a transformant carrying the plasmid only without vgb while the other control represents parental cells.
The optimum growth conditions were not totally identical to these for the enzyme production.
PH 7 and agitation rate of 150 rpm were common for both measurements.
However the optimum temperature for growth was approximately 37°C while that for P-galactosidase synthesis was approximately 42°C.
The latter temperature gave almost five fold higher rate of enzyme production over the 37°C.
The bacterial growth was relatively stable within a range of 0.08-0.5 % NaCl supplement while the enzyme synthesis was severely repressed.
The effect of five different nitrogen sources (three organic and two inorganic) was also investigated either by using various concentrations of each nitrogen supplement or under fixed concentration of different nitrogen sources.
It was found that all used nitrogen sources either induce or have little effects on bacterial growth.
In contrast the p-galactosidase production was repressed in all Enterobacter strains through more severely in presence of inorganic nitrogen supplements.
The VHb protein in the transformed Enterobacter pUC8:16 strains had shown a protective actions by minimizing the enzyme repression caused by salt or nitrogen supplements.
Main Subjects
No. of Pages
78
Table of Contents
Table of contents.
Abstract.
Chapter One : statement of the problem.
Chapter Two : material and methods.
Chapter Three : finding.
Chapter Four : discussion, conclusions and recommendations.
References.
American Psychological Association (APA)
al-Shurafa, Khalid Yusuf Ahmad. (2004). Factors affecting the production of β-galactosidase in different strains of enterobacter aerogenes. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-297693
Modern Language Association (MLA)
al-Shurafa, Khalid Yusuf Ahmad. Factors affecting the production of β-galactosidase in different strains of enterobacter aerogenes. (Master's theses Theses and Dissertations Master). Mutah University. (2004).
https://search.emarefa.net/detail/BIM-297693
American Medical Association (AMA)
al-Shurafa, Khalid Yusuf Ahmad. (2004). Factors affecting the production of β-galactosidase in different strains of enterobacter aerogenes. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-297693
Language
English
Data Type
Arab Theses
Record ID
BIM-297693
