Molecular detection of β-lactamase genes (tem and shv) in escherichia coli O157 : H7 isolated from human and animals
Other Title(s)
الكشف الجزيئيلجينات البيتالاكتاميز (tem and shv) في جرثومة الاشرشيا القولونية (O157 : H7) المعزولة من الإنسان و الحيوان
Dissertant
Thesis advisor
Khudayr, Bassam Yasin
Abbas, Basil Abd al-Zahrah
Comitee Members
Khidr, Muhammad Hasan
al-Badran, Adnan Isa
Jabbur, Muhammad Sh.
University
University of Basrah
Faculty
Veterinary College
Department
Department of Microbiology
University Country
Iraq
Degree
Master
Degree Date
2012
English Abstract
The goal of this study was to determine the occurrence of tem and she beta-lactamases genes (extended-spectrum β-lactamase ESBL) in Escherichia coli O157 : H7 isolated from beef, stool andcttale’s raw milk During a period of eight months (August 2011 to March 2012), a total of 675 samples were collected from 225stool hospitalized children suffering from diarrhea, 225beef samples collected from three regions in carcass (shoulders, abdomen and back) and 225 cttale’s raw milk samples collected from different regions in Basrah city (Al-Ashar, Al-Basrah and Al- Hadi).All specimens were screened for the presence of E.
coli and EHEC by cultured on MAC, EMB and SMAC.
A total of 256 (37.9 %) of suspected E.
coli isolates were obtained : 67 (29.8 %) from beef samples and 134 (59.6 %) from stool samples and 55 (24.4 %) from raw milk samples.
The study showed higher occurrence of E.
coli in stool samples followed by beef and milk.
All suspected E.
coli is olateswere tested biochemically and 193(75.4 %) were confirmed as E.
coli.
E.
coli isolates (193) were screened on SMAC to detect NSFEC.
Twenty nine NSFEC (67.4 %) out of 43 isolates were detected in beef samples, 44(38.9 %) out of 113 isolates were detected in stool samples and 18 (48.6 %) out of 37 isolates were detected in raw milk samples.
Latex agglutination test was used to detect serotype O157 : H7 in non- sorbitol fermenting isolate, found that : 2 (0.9 %) isolates from beef, 1(0.4 %) isolate from stool and 1(0.4 %) isolate from raw milk samples.
Four E.
coli O157 : H7 isolates were tested for their antibiotic resistance against 14 antibiotics by the Kirby-Bauer disk diffusion test.
All the isolates were found to be resistant to at least 7 antibiotics to which they were subjected.
Therefore, all these four isolates were considered to be multidrug resistant.
The E.
coli O157 : H7 β-lactamase-producing isolates(4)were further examined by PCR technique using two pairs of primers to amplify both tem and she genes.
The results revealed that all (100 %) of the isolates were positive for tagine, 2 (50 %) isolates yielded amplification products with she-PCR specific primers and2 (50 %) isolates were identified as carrying both temandshvgenes.
Main Subjects
Topics
No. of Pages
111
Table of Contents
Table of contents.
Abstract.
Chapter One : introduction.
Chapter Two : review of literatures.
Chapter Three : materials and methods.
Chapter Four : results.
Chapter Five : discussion.
Conclusion and recommendation.
References.
American Psychological Association (APA)
Khalil, Khulud Abd al-Razzaq. (2012). Molecular detection of β-lactamase genes (tem and shv) in escherichia coli O157 : H7 isolated from human and animals. (Master's theses Theses and Dissertations Master). University of Basrah, Iraq
https://search.emarefa.net/detail/BIM-317380
Modern Language Association (MLA)
Khalil, Khulud Abd al-Razzaq. Molecular detection of β-lactamase genes (tem and shv) in escherichia coli O157 : H7 isolated from human and animals. (Master's theses Theses and Dissertations Master). University of Basrah. (2012).
https://search.emarefa.net/detail/BIM-317380
American Medical Association (AMA)
Khalil, Khulud Abd al-Razzaq. (2012). Molecular detection of β-lactamase genes (tem and shv) in escherichia coli O157 : H7 isolated from human and animals. (Master's theses Theses and Dissertations Master). University of Basrah, Iraq
https://search.emarefa.net/detail/BIM-317380
Language
English
Data Type
Arab Theses
Record ID
BIM-317380
