Production of bioactive metabolites from bacillus sp.7B1(al-Mazar Karak)‎

Other Title(s)

إنتاج الايضيات النشطة بيولوجيا من Bacillus sp. 7B1 (المزار الكرك)‎

Dissertant

al-Sarayirah, Hashim

Thesis advisor

al-Zaraini, Wail Ali Hasan
al-Tarawinah, Khalid Ahmad Jafar

Comitee Members

al-Shakanbah, Jumah Muti
al-Shahatit, Sulhi Faysal
Atruz, Umar Muhammad Falih

University

Mutah University

Faculty

Faculty of Science

Department

Department of Biological Sciences

University Country

Jordan

Degree

Master

Degree Date

2013

English Abstract

In this study 10 bacterial isolates were screened for bioactive metabolites production.

Three bacterial isolates (1C1, 2C1, and 7B1) exhibit activity at least against one type of tested bacterial strains.

7B1 was selected for further study as it produce bioactive extract against the Gram positive bacterial strains, B.

subtili, M.

luteus, and S.

aureus.

Bacillus sp.

7B1 was cultivated in large scale (20L) and the produced extract was purified by chromatography.

The purified compound (band 2) was evaluated for its antibacterial activity.

Bioactivity was measured by agar diffusion and serial dilution assay.

Gram negative bacteria were resistant to the applied pure compound but Gram positive bacteria were susceptible in a concentration range between 25 μg and 100 μg.

Main Subjects

Biology

Topics

• Biotechnology
• Antibiotics

No. of Pages

30

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-322629