تأثير نوع المخفف و فترات الخزن على بعض صفات السائل المنوي في الكباش العواسيةٌ

Abstract EN

The objective of this study was to evaluate some diluents and effects of type of diluents and preservation periods of Awassi ram semen.

The diluents included egg yolk-sodium citrate, Tris fructose –egg yolk ,Skim milk-egg yolk and new modified diluents.

Dilution rate was (1:10) and semen samples were collected with artificial vagina , one ejaculate per week for 3 months (September , October, and November) in the breeding season and preserved at 5oC for 4 days to evaluated the changes in daily spermatozoa, Individual motility , percentage of alive sperm, pH, and sperm abnormalities.

Total ejaculates were collected from 6 Awassi Rams with weight mean 54 kg and aged 1.5- 2 years.

Results of the present study showed: 1- Tris fructose – egg yolk diluent was preserved significantly (p ≤ 0,01) to the sperms in semen's diluent of Awassi Rams and was superior (p ≤ 0,01) than other diluents in spermatozoa individual motility, percentage of alive sperm than other dilutions.

2- Tris fructose – egg yolk and skim milk – egg yolk diluents were less significant (p ≤ 0,01) with degree of sperms abnormality in diluted semen than Sodium citrate (SC) and modified dilutions .

3- Decrease the efficiency of Modified dilution for preserving the sperms of diluted semen with keeping good level of acidity (pH) than other dilutions.

4- The preservation periods had a significant effect on diluted and cooling semen characteristics, however the Tris fructose – egg yolk was superior dilution for preserving Awassi semen at 5oC for 4 days.