الكشف عن فيروس موزاييك البرسيم Alafalfa mosaic virus في نباتات فلفل مصابة و تقدير البروتين الكلي لأصناف مختلفة من نبات الفلفل Capsicum annuum L. معداة بنفس الفيروس

Abstract EN

Mosaic symptoms were observed on pepper plants in Riyadh region (Wasil Melds), Saudi Arabia.

Out of 16 plant species mechanically inoculated with sap prepared from leaves of infected pepper plants in 0.1 M potassium phosphate buffer, pH 7.0, 13 plant species were infected and exhibited external symptoms.

These included: Chenopodlum amaranticolor Coste and Ryen., C.

qumo&, Capsicum annuum L Datura stramonium, Somphrena globosa L, Laetuea romana, Lycoperslcon esculentum Mill., cultivar Sultana, Medlcago sativa L, Solatium melongena L.

cultivar Black beauty, Solanum nigrum, Nlcotiana glutinosa L, N.

tabaccum, and Phaseolus vulgaris L.

cultivar Green beans.

Cucurbits pepo L, and Cucumls sativus L.

did not show symptoms.

The properties of virus in the infective sap were as follows: Thermal inactlvatlon point (TIP) was between 60-70° C, dilution end point (DEP) was between 103 • 10" and the longevity In Vitro (LIV) was between 2-4 days.

ELISA indicated that the virus was alfalfa mosaic virus (AMV).

Inoculation of some pepper cultlvars with this AMV Isolate resulted in external virus symptoms which were variable among the different inoculated pepper cultlvars.

Determination of the total protein in the different pepper cultlvars revealed higher protein contents in the inoculated cultlvars compared to those which were not inoculated with AMV.

Seven plant species (Mcotiana glutinosa L, Datura stramonium L, Chenopodlum amaranticolor Coste and Ryen, Gomphrena globosa L, Capsicum annuum L, Solanum nigrum I., Phaseolus vulgaris I.) out of 11 plant species mechanically inoculated with the virus isolate, and showed AMV-llke symptoms on them ELISA indicated that the naturally infected pepper plant as well as the seven inoculated host plants that showed different symptoms, and were all positive to AMV.

Four pepper varieties were infected with the virus and that the effect of the virus varied for the different cultlvars with respect to the type and severity of symptoms expressed on them and which included mosaic, yellow mosaic, calico, mottle, leaf curl and deformation, cupping, and necrosis of the interveinal areas of lamina in some pepper cultlvars.

ELISA also indicated that it Is not necessary that the severity of symptoms and virus concentration in the infected plants are always directly proportional.