تأثير إضافة بعض المواد النباتية الطبيعية في التلوث و تكوين الأجنة الخضرية لنخيل التمر صنف الأشقر المكثرة خارج الجسم الحي

Abstract EN

This study has been performed at tissue culture lab.–Date Palm Research Center / Basrah university to determine the effect of some natural plant on the growth development of embryogenic callus and somatic embryos.

Embryogenic callus of 6 months old of Ashkar cultivar was used for this study.

This embryogenic callus was cultured on Ms medium, supplemented with the following plants materials : 1-Corn seed at conc.

12.5 and 25 g / l.

2Jujube leaves at conc.

1.25 and 2.5 g / l.

The results of this study can be summarized as follow : 1-It was found that type of medium had significant effect on the average of embryogenic callus fresh weight after 30 and 60 days from culturing.

The corn seed medium had the highest average in contrast with other media, the fresh weight were 476 and 689 mg after 30 and 60 days of culture, respectively.

2-The type of the media varied in their effect on contamination percentage of embryogenic callus after 60 days from culturing the medium with Eucalyptus leaves extract at con.

12.5 and 25, 1L had no contamination (0 %).

3-The culturing of embryogenic callus on Ms media with corn seed led to significant increase in the number of embryos which was 232 and 197 embryos for each conc.

; respectively, while the number of embryos was decreased and reached to 54 embryos in the treatment of Eucalyptus leaves at conc.

12.5 g / l.