In vitro micropropagation of dracaena sanderiana sander ex mast : an important indoor ornamental plant

Abstract EN

-A protocol has been developed for in vitro plant regeneration from a nodal explant of Dracaena sanderiana Sander ex Mast.

Nodal explant showed high callus induction potentiality on MS medium supplemented with 6.78 lM 2,4-dichlorophenoxyacetic acid (2,4-D) followed by 46.5 lM chlorophenoxy acetic acid (CPA).

The highest frequency of shoot regeneration (85%) and number of shoots per explant (5.6) were obtained on medium supplemented with 7.84 lM N6-benzylaminopurine (BA).

Rooting was high on MS solid compared to liquid medium when added with 7.38 lM indole-3-butyric acid (IBA).

Fifty percent of the roots were also directly rooted as microcuttings on soil rite, sand and peat mixture (1:1:1).

In vitro and ex vitro raised plantlets were used for acclimatization.

More than 90% of the plantlets was successfully acclimatized and established in plastic pots.

Ex vitro transferred plantlets were normal without any phenotypic aberrations.