استعمال النواتج الأيضية لبكتيريا حامض اللاكتيك العلاجية لتثبيط نمو عزلات مرضية معزولة من حيوانات مصابة

Abstract EN

This study aimed to detect metabolites inhibition activity for three therapeutic Lactic acid bacteria Lactobacillus acidophilus (Lb.

acid.) , Lactobacillus reuteri (Lb.

reut.) and Bifidobacterium ssp.

(Bif.) which were grown in whole milk and Man Reqosa and Sharp (MRS) broth as single or double (Lb.

acid.

+ Bif.) or mixed (Lb.

reut + Bif.

+ Lb.

acid) culture Cell Free Extract (CFE) of these culture which contained their metabolites of Lactic acid bacteria (exclusion Lb.

reut.) showed good inhibition against four species of pathogenic Escherichia coli ; Salmonella typhimurium ; Proteus volgaris and Staphylococcus aureus.

inhibition activity against E.

coli culture showed that 72 hours was the best incubation time for Lactic acid bacteria to produce more metabolite with best inhibition activity.

Metabolite (CFE) of Lb.

Acid culture grown in milk and MRS broth showed the best inhibition activity against all of Gram Positive and Gram negative test bacteria with over all inhibition zone diameter was 14.6 and 15.3 mm for milk and MRS broth supernatant respectively and this activity remained for more than one week as inhibition zone diameter were 13.4 and 14.5 mm for supernatant of milk and MRS respectively.