فيروس موزاييك و زوائد البازلاء-1 : مداه العائلي، تنقيته، تفاعلاته السيرولوجية، طرق انتقاله و انتشاره على المحاصيل البقولية في سورية

Abstract EN

A virus disease of lentil, characterized by rolling and mottling of leaves with tip wilting or necrosis and stunted growth, was observed in lentil fields in southern Syria since 1994.

Disease incidcncc was relatively high, almost annually, in lentil fields in Dara’a Govcmoratc.

Transmission tests showed that the disease agent can be transmitted mechanically from lentil to lentil, pea and faba bean plants and by aphids (Acyrthosiphon pisum Harris and Myziis persicae Sulzcr) in a persistent manner.

SDS-polyacrylamidc gel electrophoresis followed by Western blot showed that the virus capsid protein size was 22 kDa.

Serological data, using tissue-blot immunoassay (TBIA) and Western-blot indicated that the virus is scrologically similar to Pea enation mosaic virus-1 (PEMV-1, genus Enamovirus, family Luleoviridae).

On the basis of host range, symptoms, transmission characteristics and serology, the vims was identified as PEMV-1.

The virus was purified from systemically infected peas and yielded 1-2 mg of purified virus per one Kilogram of infected tissue.

Surveys conducted during 1997-2001 showed that PEMV-1 was widely distributed in the major lentil-growing areas of Syria, with virus incidence reaching more than 50% in some locations.

Results revealed that infection with PEMV-1 caused yield losses in all lentil and pea genotypes.

However, the extent of yield losses varied from 16% in ILL 7706 to 50% in ILL 6031.