انتقال فيروس تلون بذور الفول (BBSV)‎ في بذور مدخلات وراثية مختلفة من العدس و علاقته بحجم البذرة

Abstract EN

One hundred lentil genotypes obtained from ICARDA Gene Bank, originating from 26 countries, were evaluated for their reaction to a Syrian isolate (SV173-85) of Broad bean stain virus (BBSV, genus Comovirus, Family Comoviridae), and their ability to transmit BBSV in their seeds.

Plants were mechanically inoculated with the virus before flowering stage, during 1999/2000 growing season.

Randomized samples of genotypes were tested for BBSV by tissue-blot immunoassay (TBIA), 20 days after inoculation.

Results showed that seven genotypes were not infected with BBSV, 9 had an infection level of 1-10%, 22 had an infection level of 11-25%, 34 had an infection level of 26-50%, and 28 had an infection level of more than 50%.

After germinating embryo axes of seeds collected from BBSV–infected lentil mother plants, they were tested for the presence of BBSV in groups of 20 seedlings by the tissue-blot immunoassay.

The BBSV seed transmission rate varied between 0% and 22.4% in the genotypes evaluated.

The virus was not seed-transmitted in 10 genotypes, whereas seed transmission rate varied from 0.1 to 5% in 28 genotypes, 5.1 to 10% in 28 genotypes, 10.1 to 15% in 20 genotypes, and more than 15% in 7 genotypes.

There was no significant correlation between seed size and seed transmission