إخلاف النباتات و حفظ كالس أصول الحمضيات المستحث من زراعة البويضات غير البالغة خارج الجسم الحي

Abstract EN

Assays were performed to obtain embryogenic lines from four citrus rootstock bv in vitro culture of ovules collected from immature fruits 20-60 days after anthesis.

Three months alter inoculation, embryogenic callus was obtained from sweet orange and Cleopatra mandarin ovules (40 day after anthesis) that cultured on MS medium supplemented with 6% sucrose and llmg/L kinetin.

Following five subcultures, this callus became habituated to kinetin.

After subculture in MT medium (6% sucrose) with intervals of two months for about two vears, such callus line maintained an embryogenic potential and without chromosome number variation (2n=2x=18).

The effective media for producing embryoids from cailli was MT medium modified with 6.8%lactose.