Detection of extended–spectrum beta lactamase in bacteria isolated from urine of pregnant women

Abstract EN

The aim of this study was to detect extended spectrum β- lactamase (ESBL) in bacteria isolated from urine collected from urinary tract of infected pregnant women.

A total of 100 midstream urine samples were collected from Omdurman Military Hospital from April to May2013.

Specimens were cultured onto cysteine- lactose- electrolyte-deficient (CLED) agar to identify urinary tract infection (UTI) causative bacteria by colonial morphology, Gram reaction and conventional biochemical tests.

Out of 100 urine specimen; 56(56%) of samples gave significant bacterial growth (the Gram negative bacilli were 42(75%).

The most frequent Gram negative were Klebsiella oxytoca 15 (26.8%) and Escherichia coli 10(17.9%), while the most abundant Gram positive was Staphylococcus saprophyticus.

In The susceptibility test, 5 isolates were resistant to 3rdgeneration cephalosporins (ceftazidime, ceftriaone and cefotaxime) as follow: 2 K.

pneumoniae, 2 E.

coli and 1 Proteus vulgaris.

ESBL production test done for all bacterial resistance to 3rd generation cephalosporin and then confirmed by combination test by using Double disc synergy test 5(11.9% ) isolates gave positive result.

The PCR was done for 5 isolates using TEM, SHV, and CTX-M primers.TEM gene was the most predominant one followed by CTX-M and the least one was SHV gene.

From the results it could be concluded that these genes are responsible for the resistance of cephalosporins.