An indirect atomic absorption spectrophotometric determination of cefotaxime in pharmaceutical formulations by using rhodium (II)‎ as a mediating metal

Abstract EN

A new application of an indirect atomic absorption spectrometric (AAS) method was offered for the assay of low concentrations of cefotaxime (CFX) in pharmaceutical in pure and pharmaceutical dosage form with good accuracy and precision.

The method is depended on the formation of chelating complex between the drug cefotaxime and rhodium(II) to form orange-yellowish product.

All experimental parameters such as, pH, concentration of rhodium, reaction time, extraction time and phase ratio have been investigated.

The linearity (2-50) μg mL-1, detection limit (S/N) of 0.13 μg mL-1, accuracy as the %Erel of 1.59, and recoveries ranged from 101.2- 101.87%, with mean value of 101.59±0.73.

The proposed method was applied for the determination of CFX in the drug Sefotak by both direct calibration and standard additions procedures and found to be 989.87 and 970.93 mg per unite, respectively compared with the stated value of 1000 mg per unite.

This method is also compared statistically with direct determination by using UV-VIS spectrophotometric technique which is preformed in our laboratory and found to be insignificant at 95% confidence level.

All statistical calculations were implemented via the chemsoftware Minitab version 11.