الكشف عن جينات مقاومة للفانكومايسين في المكورات العنقودية الذهبية المعزولة من عينات سريرية و بيئية في محافظة ذي قار

Abstract EN

One handred of S.aureus were isolated from 650 of clinical and environmental samples.

The clinical samples were 470 and environmental were 180.

Eighty five of clinical isolats were isolated from burn swabs, ear swabs, nasal swabs, wound swabs, vaginal swabs and urine samples.Antibiotic suceptibity was done for the isolates a gainst (13) antibiotics by disk diffusion methed.

The results showed variations in the resistance .

Minimum inhibitory concentration (MIC) for vancomycin was detected for 20 isolates , (10 ) from sensitive isolates and 10 from resistante using VITEK2 apparatus .

MIC was ranged from ( 1-32 mg/mlc).

The DNA was extracted from the isolates and nucgene which uniqe for S.aureus was detected in all the isolates through the appearance of gene band on the gel.

Polymerase .

chain reactions for detection of vancomycin resistance genes ( van A , van X , van H, van HAX ) was done usiny specific primers.

PCR Products separated on 2% a garos .

The results showed that 9 isolates have van X, 6 isolates have van H, 2 isolates have van A while van HAX was showed in only one isolates.