القدرة التضادية لبعض عزلات من Bacillus spp. إزاء الكائن الممرض المسبب لمرض ذبول العدس الوعائي Fusarium oxysporum f. sp. lentis

Abstract EN

Thirty five bacterial isolates of Bacillus spp..

with antagonistic activity rates ranging from 52 to 77% against Fusarium oxysporum f.

sp.

lentis were identified.

Six isolates were tested, singly or in combination, for their in-vivo antagonistic effect in soil infested with Fusarium oxysporum f.

sp.

lentis under plastic house (pots) and field conditions (sick plot).

Two lentil lines: ILL 4605 (highly susceptible) and ILL 7136 (moderately susceptible) were used.

In-vitro confrontations were made between the antagonists and three rhizobia specific to lentil for possible interaction.

In-vitro test was also conducted to study the effects of commonly used seed dressing fungicides on the growth of antagonists.

Results indicated that seed germination rate was not affected by coating lentil seeds with the antagonists.

There was a significant genotype by treatment interaction for percent wilted plants taken 127 and 136 days after planting.

The antagonists did not affect the growth of three lentil specific rhizobia strains.

Moreover, their growth was not affected by the two commonly used seed dressing fungicides (benomyl and thiabendazole).

From the above, their is good potential in the use of selected Bacillus spp.

for the control of lentil vascular wilt, which is an indication of their potential role in the biological and integrated control of lentil wilt.