الكشف عن فيروس تقزم فول الصويا في بعض المحاصيل البقولية الغذائية بواسطة اختبارات اليزا المختلة

Abstract EN

Soybean dwarf luteovirus (SbDV) was detected by DAS-ELISA and TAS-ELISA in lentil, faba bean and pea by using different polyclonal and monoclonal antibodies.The highest concentration of the virus wras found in faba bean and lentil stems at ten days after inoculation.

When different Enzyme linked immunosorbent assay (ELISA) variants were compared, TAS-ELISA was the most sensitive.

To improve detection efficiency further many extraction buffers were compared.

It was possible to shorten the TAS-ELISA procedure to six hours without significant loss in its detection sensitivity.

The virus w'as detected in A.

pisum when extracted in 0.2M phosphate buffer.