Optical Spectroscopy for Noninvasive Monitoring of Stem Cell Differentiation
Joint Authors
Elfick, Alistair
Downes, Andrew
Mouras, Rabah
Source
Journal of Biomedicine and Biotechnology
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-02-16
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
There is a requirement for a noninvasive technique to monitor stem cell differentiation.
Several candidates based on optical spectroscopy are discussed in this review: Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and coherent anti-Stokes Raman scattering (CARS) microscopy.
These techniques are briefly described, and the ability of each to distinguish undifferentiated from differentiated cells is discussed.
FTIR spectroscopy has demonstrated its ability to distinguish between stem cells and their derivatives.
Raman spectroscopy shows a clear reduction in DNA and RNA concentrations during embryonic stem cell differentiation (agreeing with the well-known reduction in the nucleus to cytoplasm ratio) and also shows clear increases in mineral content during differentiation of mesenchymal stem cells.
CARS microscopy can map these DNA, RNA, and mineral concentrations at high speed, and Mutliplex CARS spectroscopy/microscopy is highlighted as the technique with most promise for future applications.
American Psychological Association (APA)
Downes, Andrew& Mouras, Rabah& Elfick, Alistair. 2010. Optical Spectroscopy for Noninvasive Monitoring of Stem Cell Differentiation. Journal of Biomedicine and Biotechnology،Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-446414
Modern Language Association (MLA)
Downes, Andrew…[et al.]. Optical Spectroscopy for Noninvasive Monitoring of Stem Cell Differentiation. Journal of Biomedicine and Biotechnology No. 2010 (2010), pp.1-10.
https://search.emarefa.net/detail/BIM-446414
American Medical Association (AMA)
Downes, Andrew& Mouras, Rabah& Elfick, Alistair. Optical Spectroscopy for Noninvasive Monitoring of Stem Cell Differentiation. Journal of Biomedicine and Biotechnology. 2010. Vol. 2010, no. 2010, pp.1-10.
https://search.emarefa.net/detail/BIM-446414
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-446414