Potential Novel Biomarkers for Diabetic Testicular Damage in Streptozotocin-Induced Diabetic Rats : Nerve Growth Factor Beta and Vascular Endothelial Growth Factor
Joint Authors
Kiray, Muge
Camsari, Ulas Mehmet
Baykara, Basak
Aksu, Ilkay
Sisman, Ali Rıza
Uysal, Nazan
Evren, Merve
Ates, Mehmet
Guvendi, Guven
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-03-20
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
It is well known that diabetes mellitus may cause testicular damage.
Vascular endothelial growth factor (VEGF) and nerve growth factor beta (NGF-β) are important neurotrophic factors for male reproductive system.
Objective.
We aimed to investigate the correlation between testicular damage and testicular VEGF and NGF-β levels in diabetic rats.
Methods.
Diabetes was induced by streptozotocin (STZ, 45 mg/kg/i.p.) in adult rats.
Five weeks later testicular tissue was removed; testicular VEGF and NGF-β levels were measured by ELISA.
Testicular damage was detected by using hematoxylin and eosin staining and periodic acid-Schiff staining, and apoptosis was identified by terminal-deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL).
Seminiferous tubular sperm formation was evaluated using Johnsen’s score.
Results.
In diabetic rats, seminiferous tubule diameter was found to be decreased; basement membrane was found to be thickened in seminiferous tubules and degenerated germ cells.
Additionally, TUNEL-positive cells were increased in number of VEGF+ cells and levels of VEGF and NGF-β were decreased in diabetic testes.
Correlation between VEGF and NGF-β levels was strong.
Conclusion.
These results suggest that the decrease of VEGF and NGF-β levels is associated with the increase of the apoptosis and testicular damage in diabetic rats.
Testis VEGF and NGF-β levels could be potential novel biomarkers for diabetes induced testicular damage.
American Psychological Association (APA)
Sisman, Ali Rıza& Kiray, Muge& Camsari, Ulas Mehmet& Evren, Merve& Ates, Mehmet& Baykara, Basak…[et al.]. 2014. Potential Novel Biomarkers for Diabetic Testicular Damage in Streptozotocin-Induced Diabetic Rats : Nerve Growth Factor Beta and Vascular Endothelial Growth Factor. Disease Markers،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-447039
Modern Language Association (MLA)
Sisman, Ali Rıza…[et al.]. Potential Novel Biomarkers for Diabetic Testicular Damage in Streptozotocin-Induced Diabetic Rats : Nerve Growth Factor Beta and Vascular Endothelial Growth Factor. Disease Markers No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-447039
American Medical Association (AMA)
Sisman, Ali Rıza& Kiray, Muge& Camsari, Ulas Mehmet& Evren, Merve& Ates, Mehmet& Baykara, Basak…[et al.]. Potential Novel Biomarkers for Diabetic Testicular Damage in Streptozotocin-Induced Diabetic Rats : Nerve Growth Factor Beta and Vascular Endothelial Growth Factor. Disease Markers. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-447039
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447039