Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives
Joint Authors
Nirmal, Nilesh P.
Laxman, R. Seeta
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-07-03
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A fungal strain (Conidiobolus brefeldianus MTCC 5184) isolated from plant detritus secreted a high activity alkaline protease.
Thermostability studies of the fungal alkaline protease (FAP) revealed that the protease is stable up to 50°C with 40% residual activity after one hour.
Effect of various additives such as sugars, sugar alcohols, polyols, and salts, on the thermostability of FAP was evaluated.
Among the additives tested, glycerol, mannitol, xylitol, sorbitol, and trehalose were found to be very effective in increasing the stability of FAP, which was found to be concentration dependent.
Fivefold increase in residual activity of FAP was observed in the presence of trehalose (50%) and sorbitol (50%) at 50°C for 4 h, compared to FAP without additive.
Other additives like calcium at 20 mM and 10–15% ammonium sulphate showed lower stability improvement than trehalose and sorbitol.
NaCl, MgCl2, K2HPO4, and glycine were found to be poor stabilizers and showed only a marginal improvement.
PEG 6000 did not show any increase in stability but was found to be slightly inhibitory.
American Psychological Association (APA)
Nirmal, Nilesh P.& Laxman, R. Seeta. 2014. Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives. Enzyme Research،Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-447142
Modern Language Association (MLA)
Nirmal, Nilesh P.& Laxman, R. Seeta. Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives. Enzyme Research No. 2014 (2014), pp.1-8.
https://search.emarefa.net/detail/BIM-447142
American Medical Association (AMA)
Nirmal, Nilesh P.& Laxman, R. Seeta. Enhanced Thermostability of a Fungal Alkaline Protease by Different Additives. Enzyme Research. 2014. Vol. 2014, no. 2014, pp.1-8.
https://search.emarefa.net/detail/BIM-447142
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447142