Role of Peroxisome Proliferator-Activated Receptor βδ and B-Cell Lymphoma-6 in Regulation of Genes Involved in Metastasis and Migration in Pancreatic Cancer Cells
Joint Authors
Coleman, Jeffrey D.
Thompson, Jerry T.
Smith, Russell W.
Vanden Heuvel, John P.
Prokopczyk, Bogdan
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-05-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
PPARβ/δ is a ligand-activated transcription factor that regulates various cellular functions via induction of target genes directly or in concert with its associated transcriptional repressor, BCL-6.
Matrix remodeling proteinases are frequently over-expressed in pancreatic cancer and are involved with metastasis.
The present study tested the hypothesis that PPARβ/δ is expressed in human pancreatic cancer cells and that its activation could regulate MMP-9, decreasing cancer cells ability to transverse the basement membrane.
In human pancreatic cancer tissue there was significantly higher expression of MMP-9 and PPARβ/δ, and lower levels of BCL-6 mRNA.
PPARβ/δ activation reduced the TNFα-induced expression of various genes implicated in metastasis and reduced the invasion through a basement membrane in cell culture models.
Through the use of short hairpin RNA inhibitors of PPARβ/δ, BCL-6, and MMP-9, it was evident that PPARβ/δ was responsible for the ligand-dependent effects whereas BCL-6 dissociation upon GW501516 treatment was ultimately responsible for decreasing MMP-9 expression and hence invasion activity.
These results suggest that PPARβ/δ plays a role in regulating pancreatic cancer cell invasion through regulation of genes via ligand-dependent release of BCL-6 and that activation of the receptor may provide an alternative therapeutic method for controlling migration and metastasis.
American Psychological Association (APA)
Coleman, Jeffrey D.& Thompson, Jerry T.& Smith, Russell W.& Prokopczyk, Bogdan& Vanden Heuvel, John P.. 2013. Role of Peroxisome Proliferator-Activated Receptor βδ and B-Cell Lymphoma-6 in Regulation of Genes Involved in Metastasis and Migration in Pancreatic Cancer Cells. PPAR Research،Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-447371
Modern Language Association (MLA)
Coleman, Jeffrey D.…[et al.]. Role of Peroxisome Proliferator-Activated Receptor βδ and B-Cell Lymphoma-6 in Regulation of Genes Involved in Metastasis and Migration in Pancreatic Cancer Cells. PPAR Research No. 2013 (2013), pp.1-11.
https://search.emarefa.net/detail/BIM-447371
American Medical Association (AMA)
Coleman, Jeffrey D.& Thompson, Jerry T.& Smith, Russell W.& Prokopczyk, Bogdan& Vanden Heuvel, John P.. Role of Peroxisome Proliferator-Activated Receptor βδ and B-Cell Lymphoma-6 in Regulation of Genes Involved in Metastasis and Migration in Pancreatic Cancer Cells. PPAR Research. 2013. Vol. 2013, no. 2013, pp.1-11.
https://search.emarefa.net/detail/BIM-447371
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447371