Evaluation of Handheld Assays for the Detection of Ricin and Staphylococcal Enterotoxin B in Disinfected Waters
Joint Authors
Shah, Saumil S.
Insalaco, Joseph M.
Biggs, Tracey D.
Conley, Edward K.
Zulich, Alan W.
Schenning, Amanda M.
Reilly, Lisa M.
Wade, Mary Margaret
Bevilacqua, Vicky L. H.
Emanuel, Peter A.
Neuendorff, Lisa K.
Source
International Journal of Microbiology
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-14
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Abstract EN
Development of a rapid field test is needed capable of determining if field supplies of water are safe to drink by the warfighter during a military operation.
The present study sought to assess the effectiveness of handheld assays (HHAs) in detecting ricin and Staphylococcal Enterotoxin B (SEB) in water.
Performance of HHAs was evaluated in formulated tap water with and without chlorine, reverse osmosis water (RO) with chlorine, and RO with bromine.
Each matrix was prepared, spiked with ricin or SEB at multiple concentrations, and then loaded onto HHAs.
HHAs were allowed to develop and then read visually.
Limits of detection (LOD) were determined for all HHAs in each water type.
Both ricin and SEB were detected by HHAs in formulated tap water at or below the suggested health effect levels of 455 ng/mL and 4.55 ng/mL, respectively.
However, in brominated or chlorinated waters, LODs for SEB increased to approximately 2,500 ng/mL.
LODs for ricin increased in chlorinated water, but still remained below the suggested health effect level.
In brominated water, the LOD for ricin increased to approximately 2,500 ng/mL.
In conclusion, the HHAs tested were less effective at detecting ricin and SEB in disinfected water, as currently configured.
American Psychological Association (APA)
Wade, Mary Margaret& Biggs, Tracey D.& Insalaco, Joseph M.& Neuendorff, Lisa K.& Bevilacqua, Vicky L. H.& Schenning, Amanda M.…[et al.]. 2011. Evaluation of Handheld Assays for the Detection of Ricin and Staphylococcal Enterotoxin B in Disinfected Waters. International Journal of Microbiology،Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-448280
Modern Language Association (MLA)
Wade, Mary Margaret…[et al.]. Evaluation of Handheld Assays for the Detection of Ricin and Staphylococcal Enterotoxin B in Disinfected Waters. International Journal of Microbiology No. 2011 (2011), pp.1-5.
https://search.emarefa.net/detail/BIM-448280
American Medical Association (AMA)
Wade, Mary Margaret& Biggs, Tracey D.& Insalaco, Joseph M.& Neuendorff, Lisa K.& Bevilacqua, Vicky L. H.& Schenning, Amanda M.…[et al.]. Evaluation of Handheld Assays for the Detection of Ricin and Staphylococcal Enterotoxin B in Disinfected Waters. International Journal of Microbiology. 2011. Vol. 2011, no. 2011, pp.1-5.
https://search.emarefa.net/detail/BIM-448280
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448280