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A “Novel” Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (Cichorium intybus L., Asteraceae)
Joint Authors
Hilbert, Jean-Louis
Hendriks, Theo
Grec, Sébastien
Quillet, Marie-Christine
Bahri, Meriem
Trotin, Francis
Hance, Philippe
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-05
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Medicine
Information Technology and Computer Science
Abstract EN
A “novel” protocol is presented for easy and reliable estimation of soluble hydroxycinnamate levels in Cichorium intybus L.
leaf tissue in large-scale experiments.
Samples were standardized by punching 6 discs per leaf, and hydroxycinnamates were extracted by submerging the discs in 80% ethanol with 5% acetic acid for at least 48 h in the darkness at 4°C.
Residual dry mass of the discs was used for a posteriori correction of compound levels.
Chlorophyll was eliminated by chloroform, and the aqueous phases were transferred to microplates, dried, and dissolved in 50% methanol for HPLC analysis and storage.
An HPLC program of 8 min was developed for the analysis of the extracts.
Comparisons with extractions of liquid nitrogen powders indicated that the novel extraction method was reliable.
No degradation of the major hydroxycinnamates—caftaric, chlorogenic, and chicoric acids—was observed, during maceration at ambient temperatures, or after storage for 1 year.
American Psychological Association (APA)
Bahri, Meriem& Hance, Philippe& Grec, Sébastien& Quillet, Marie-Christine& Trotin, Francis& Hilbert, Jean-Louis…[et al.]. 2012. A “Novel” Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (Cichorium intybus L., Asteraceae). The Scientific World Journal،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-449171
Modern Language Association (MLA)
Bahri, Meriem…[et al.]. A “Novel” Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (Cichorium intybus L., Asteraceae). The Scientific World Journal No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-449171
American Medical Association (AMA)
Bahri, Meriem& Hance, Philippe& Grec, Sébastien& Quillet, Marie-Christine& Trotin, Francis& Hilbert, Jean-Louis…[et al.]. A “Novel” Protocol for the Analysis of Hydroxycinnamic Acids in Leaf Tissue of Chicory (Cichorium intybus L., Asteraceae). The Scientific World Journal. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-449171
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-449171