Breast Carcinoma Progression and Tumour Vascular Markers Related to Apoptotic Mechanisms
Joint Authors
Urban, Peter
Bilecova-Rabajdova, Miroslava
Kruzliak, Peter
Fialkovicová, Viera
Marekova, Maria
Gregova, Kristina
Varga, Jan
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-02-18
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Background.
In the last few years, the cancer research had tried to identify and characterize new biochemical and molecular pathways in which the inhibition induces prosurvival mechanisms.
Our work describes the expression of two different members of apoptotic regulatory pathway and their relationship with a progression of breast carcinoma.
Materials and Methods.
We compared expression of genes related to apoptosis (DR6 and Gpm6B) in the blood of patients suffering from stage I of breast cancer in different grades (I–IV), with healthy controls.
After isolation of mRNA, transcription of mRNA into the cDNA was performed.
The quantification of gene expression changes in DR6 and Gpm6B was detected by RT-PCR method.
Analysis at the protein level was performed by the Western blot.Results.
In statistical analysis of Dr6 mRNA level changes we detected significant increase starting in Grading 1 (G1) and reached maximal level in G3.This expression on mRNA levels was similar to protein levels, which copy rising tendency with maximal value in G3.
The results of Gpm6B were significantly lower.Conclusion.
This result showed that antiapoptotic signalling during neovascularization is increased significantly.
It would be advisable in the future to study the influence of cytostatic treatment on the expression of genes related to apoptotic pathways and their relationship with progression of breast cancer tumours.
American Psychological Association (APA)
Bilecova-Rabajdova, Miroslava& Urban, Peter& Gregova, Kristina& Varga, Jan& Fialkovicová, Viera& Kruzliak, Peter…[et al.]. 2014. Breast Carcinoma Progression and Tumour Vascular Markers Related to Apoptotic Mechanisms. Disease Markers،Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-450227
Modern Language Association (MLA)
Bilecova-Rabajdova, Miroslava…[et al.]. Breast Carcinoma Progression and Tumour Vascular Markers Related to Apoptotic Mechanisms. Disease Markers No. 2014 (2014), pp.1-7.
https://search.emarefa.net/detail/BIM-450227
American Medical Association (AMA)
Bilecova-Rabajdova, Miroslava& Urban, Peter& Gregova, Kristina& Varga, Jan& Fialkovicová, Viera& Kruzliak, Peter…[et al.]. Breast Carcinoma Progression and Tumour Vascular Markers Related to Apoptotic Mechanisms. Disease Markers. 2014. Vol. 2014, no. 2014, pp.1-7.
https://search.emarefa.net/detail/BIM-450227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450227