Zinc Protoporphyrin Upregulates Heme Oxygenase-1 in PC-3 Cells via the Stress Response Pathway
Author
Source
International Journal of Cell Biology
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-02-17
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Zinc protoporphyrin IX (ZnPP), a naturally occurring molecule formed in iron deficiency or lead poisoning, is a potent competitive inhibitor of heme oxygenase-1 (HO-1).
It also regulates expression of HO-1 at the transcriptional level.
However, the effect of ZnPP on HO-1 expression is controversial.
It was shown to induce HO-1 expression in some cells, but suppress it in others.
The objective of this study is to investigate the effect of ZnPP on HO-1 expression in prostate cancer PC-3 cells.
Incubation of PC-3 cells with 10 μM ZnPP for 4 h showed only a slight induction of HO-1 mRNA and protein, but the induction was high after 16 h and was maintained through 48 h of incubation.
Of all the known responsive elements in the HO-1 promoter, ZnPP activated mainly the stress response elements.
Of the various protein kinase inhibitors and antioxidant tested, only Ro 31-8220 abrogated ZnPP-induced HO-1 expression, suggesting that activation of HO-1 gene by ZnPP may involve protein kinase C (PKC).
The involvement of PKC α, β, δ, η, θ, and ζ isoforms was ruled out by the use of specific inhibitors.
The isoform of PKC involved and participation of other transcription factors remain to be studied.
American Psychological Association (APA)
Kwok, Simon C. M.. 2013. Zinc Protoporphyrin Upregulates Heme Oxygenase-1 in PC-3 Cells via the Stress Response Pathway. International Journal of Cell Biology،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-450790
Modern Language Association (MLA)
Kwok, Simon C. M.. Zinc Protoporphyrin Upregulates Heme Oxygenase-1 in PC-3 Cells via the Stress Response Pathway. International Journal of Cell Biology No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-450790
American Medical Association (AMA)
Kwok, Simon C. M.. Zinc Protoporphyrin Upregulates Heme Oxygenase-1 in PC-3 Cells via the Stress Response Pathway. International Journal of Cell Biology. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-450790
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-450790