β-Catenin Does Not Confer Tumorigenicity When Introduced into Partially Transformed Human Mesenchymal Stem Cells
Joint Authors
Li, Nan
Gorlick, Sarah
Gill, Jonathan
Zhang, Wendong
Piperdi, Sajida
Austin-Page, Lukas
Chung, So Hak
Gorlick, Richard G.
Park, Amy
Ahluwalia, Manpreet
Geller, David S.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-18
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Although osteosarcoma is the most common primary malignant bone tumor in children and adolescents, its cell of origin and the genetic alterations are unclear.
Previous studies have shown that serially introducing hTERT, SV40 large TAg, and H-Ras transforms human mesenchymal stem cells into two distinct sarcomas cell populations, but they do not form osteoid.
In this study, β-catenin was introduced into mesenchymal stem cells already containing hTERT and SV40 large TAg to analyze if this resulted in a model which more closely recapitulated osteosarcoma.
Results.
Regardless of the level of induced β-catenin expression in the stable transfectants, there were no marked differences induced in their phenotype or invasion and migration capacity.
Perhaps more importantly, none of them formed tumors when injected into immunocompromised mice.
Moreover, the resulting transformed cells could be induced to osteogenic and chondrogenic differentiation but not to adipogenic differentiation.
Conclusions.
β-catenin, although fostering osteogenic differentiation, does not induce the malignant features and tumorigenicity conveyed by oncogenic H-RAS when introduced into partly transformed mesenchymal stem cells.
This may have implications for the role of β-catenin in osteosarcoma pathogenesis.
It also may suggest that adipogenesis is an earlier branch point than osteogenesis and chondrogenesis in normal mesenchymal differentiation.
American Psychological Association (APA)
Piperdi, Sajida& Austin-Page, Lukas& Geller, David S.& Ahluwalia, Manpreet& Gorlick, Sarah& Gill, Jonathan…[et al.]. 2012. β-Catenin Does Not Confer Tumorigenicity When Introduced into Partially Transformed Human Mesenchymal Stem Cells. Sarcoma،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-451044
Modern Language Association (MLA)
Piperdi, Sajida…[et al.]. β-Catenin Does Not Confer Tumorigenicity When Introduced into Partially Transformed Human Mesenchymal Stem Cells. Sarcoma No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-451044
American Medical Association (AMA)
Piperdi, Sajida& Austin-Page, Lukas& Geller, David S.& Ahluwalia, Manpreet& Gorlick, Sarah& Gill, Jonathan…[et al.]. β-Catenin Does Not Confer Tumorigenicity When Introduced into Partially Transformed Human Mesenchymal Stem Cells. Sarcoma. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-451044
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451044