Combined Application of 17β-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice
Joint Authors
Trotter, Andreas
Schrader, Roland Matthias
Beyer, Cordian
Kipp, Markus
Source
International Journal of Pediatrics
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-03-22
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Preterm delivery is associated with disruption of the placental supply with 17β-estradiol (E2) and progesterone (P).
The aim is to evaluate the role of E2 and P on the regulation of key proteins in lung development in embryonic lung cells.
Alveolar cell type II (AT-II) and central lung fibroblast cultures were established from mouse embryos.
Cells were exposed for 24 hours to E2 and/or P, the estrogen receptor antagonist ICI 182.780 (ICI) and the progesterone receptor antagonist mifepristone (RU 486).
The mRNA expression of vascular endothelial growth factor (VEGF) and surfactant protein B and C (SB-B, SB-C) was determined, and protein levels of VEGF were measured.
Only the combined treatment with E2 and P increased mRNA expression and VEGF protein in AT-II cells and lung fibroblasts.
Combined treatment also promoted SP-B and SP-C expression in AT-II cells.
Pretreatment with ICI and RU 486 completely abolished the E2 and P induced effects.
E2 and P enhanced expression of VEGF and surfactant proteins in primary embryonic lung cells and may be involved in regulating expression of key molecules for the prenatal lung development and postnatal lung function.
American Psychological Association (APA)
Trotter, Andreas& Kipp, Markus& Schrader, Roland Matthias& Beyer, Cordian. 2009. Combined Application of 17β-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice. International Journal of Pediatrics،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-451492
Modern Language Association (MLA)
Trotter, Andreas…[et al.]. Combined Application of 17β-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice. International Journal of Pediatrics No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-451492
American Medical Association (AMA)
Trotter, Andreas& Kipp, Markus& Schrader, Roland Matthias& Beyer, Cordian. Combined Application of 17β-Estradiol and Progesterone Enhance Vascular Endothelial Growth Factor and Surfactant Protein Expression in Cultured Embryonic Lung Cells of Mice. International Journal of Pediatrics. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-451492
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451492