TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-κB- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes
Joint Authors
Orzechowski, Arkadiusz
Pijet, Barbara
Pająk, Beata
Litwiniuk, Anna
Pijet, Maja
Gajewska, Małgorzata
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-12-17
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
TNF-α was shown to stimulate mitogenicity in C2C12 myoblasts.
Selected cytokines TNF-α, IFNα, or IFNγ reduced the expression of myosin heavy chain (MyHC IIa) when given together.
Molecular mechanisms of cytokine activities were controlled by NF-κB and JAK/STAT signaling pathways, as metabolic inhibitors, curcumin and AG490, inhibited some of TNF-α and IFNα/IFNγ effects.
Insulin was hardly antagonistic to TNF-α- and IFNα/IFNγ-dependent decrease in MyHC IIa protein expression.
Cytokines used individually or together also repressed myogenesis of C2C12 cells.
Moreover, TNF-α- and IFNα/IFNγ-dependent effects on C2C12 myotubes were associated with increased activity of Atrogin1 and MuRF1 genes, which code ubiquitin ligases.
MyHC IIa gene activity was unaltered by cytokines.
Inhibition of NF-κB or JAK/STAT with specific metabolic inhibitors decreased activity of Atrogin1 and MuRF1 but not MyHC IIa gene.
Overall, these results suggest cooperation between cytokines in the reduction of MyHC IIa protein expression level via NF-κB/JAK/STAT signaling pathways and activation of Atrogin1 and MuRF1 genes as their molecular targets.
Insulin cotreatment or pretreatment does not protect against muscle decay induced by examined proinflammatory cytokines.
American Psychological Association (APA)
Pijet, Barbara& Pijet, Maja& Litwiniuk, Anna& Gajewska, Małgorzata& Pająk, Beata& Orzechowski, Arkadiusz. 2013. TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-κB- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes. Mediators of Inflammation،Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-451571
Modern Language Association (MLA)
Pijet, Barbara…[et al.]. TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-κB- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes. Mediators of Inflammation No. 2013 (2013), pp.1-18.
https://search.emarefa.net/detail/BIM-451571
American Medical Association (AMA)
Pijet, Barbara& Pijet, Maja& Litwiniuk, Anna& Gajewska, Małgorzata& Pająk, Beata& Orzechowski, Arkadiusz. TNF-α and IFN-s-Dependent Muscle Decay Is Linked to NF-κB- and STAT-1α-Stimulated Atrogin1 and MuRF1 Genes in C2C12 Myotubes. Mediators of Inflammation. 2013. Vol. 2013, no. 2013, pp.1-18.
https://search.emarefa.net/detail/BIM-451571
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-451571