Quantitative Longitudinal Imaging of Vascular Inflammation and Treatment by Ezetimibe in apoE Mice by FMT Using New Optical Imaging Biomarkers of Cathepsin Activity and αvβ3 Integrin

Joint Authors

Groves, Kevin
Rajopadhye, Milind
Bao, Bagna
Sur, Cyrille
Bednar, Bohumil
Suresch, Donna
Connolly, Brett
Patel, Manishkumar
Lin, Shu-An
Klimas, Michael
Peterson, Jeffrey D.

Source

International Journal of Molecular Imaging

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-10-17

Country of Publication

Egypt

No. of Pages

13

Main Subjects

Medicine

Abstract EN

Inflammation as a core pathological event of atherosclerotic lesions is associated with the secretion of cathepsin proteases and the expression of αvβ3 integrin.

We employed fluorescence molecular tomographic (FMT) noninvasive imaging of these molecular activities using cathepsin sensing (ProSense, CatB FAST) and αvβ3 integrin (IntegriSense) near-infrared fluorescence (NIRF) agents.

A statistically significant increase in the ProSense and IntegriSense signal was observed within the chest region of apoE−/− mice (P<0.05) versus C57BL/6 mice starting 25 and 22 weeks on high cholesterol diet, respectively.

In a treatment study using ezetimibe (7 mg/kg), there was a statistically significant reduction in the ProSense and CatB FAST chest signal of treated (P<0.05) versus untreated apoE−/− mice at 31 and 21 weeks on high cholesterol diet, respectively.

The signal of ProSense and CatB FAST correlated with macrophage counts and was found associated with inflammatory cells by fluorescence microscopy and flow cytometry of cells dissociated from aortas.

This report demonstrates that cathepsin and αvβ3 integrin NIRF agents can be used as molecular imaging biomarkers for longitudinal detection of atherosclerosis, and cathepsin agents can monitor anti-inflammatory effects of ezetimibe with applications in preclinical testing of therapeutics and potentially for early diagnosis of atherosclerosis in patients.

American Psychological Association (APA)

Lin, Shu-An& Patel, Manishkumar& Suresch, Donna& Connolly, Brett& Bao, Bagna& Groves, Kevin…[et al.]. 2012. Quantitative Longitudinal Imaging of Vascular Inflammation and Treatment by Ezetimibe in apoE Mice by FMT Using New Optical Imaging Biomarkers of Cathepsin Activity and αvβ3 Integrin. International Journal of Molecular Imaging،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-453058

Modern Language Association (MLA)

Lin, Shu-An…[et al.]. Quantitative Longitudinal Imaging of Vascular Inflammation and Treatment by Ezetimibe in apoE Mice by FMT Using New Optical Imaging Biomarkers of Cathepsin Activity and αvβ3 Integrin. International Journal of Molecular Imaging No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-453058

American Medical Association (AMA)

Lin, Shu-An& Patel, Manishkumar& Suresch, Donna& Connolly, Brett& Bao, Bagna& Groves, Kevin…[et al.]. Quantitative Longitudinal Imaging of Vascular Inflammation and Treatment by Ezetimibe in apoE Mice by FMT Using New Optical Imaging Biomarkers of Cathepsin Activity and αvβ3 Integrin. International Journal of Molecular Imaging. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-453058

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-453058