Synthesis of Peptides from α- and β-Tubulin Containing Glutamic Acid Side-Chain Linked Oligo-Glu with Defined Length
Joint Authors
Teichmann, Aileen
Tegge, Werner
Bonafe, Carlos F. S.
Erck, Christian
Source
International Journal of Peptides
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-12-15
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
Side-chain oligo- and polyglutamylation represents an important posttranslational modification in tubulin physiology.
The particular number of glutamate units is related to specific regulatory functions.
In this work, we present a method for the synthesis of building blocks for the Fmoc synthesis of peptides containing main chain glutamic acid residues that carry side-chain branching with oligo-glutamic acid.
The two model peptide sequences CYEEVGVDSVEGEG-E(Ex)-EEGEEY and CQDATADEQG-E(Ex)-FEEEEGEDEA from the C-termini of mammalian α1- and β1-tubulin, respectively, containing oligo-glutamic acid side-chain branching with lengths of 1 to 5 amino acids were assembled in good yield and purity.
The products may lead to the generation of specific antibodies which should be important tools for a more detailed investigation of polyglutamylation processes.
American Psychological Association (APA)
Tegge, Werner& Bonafe, Carlos F. S.& Teichmann, Aileen& Erck, Christian. 2010. Synthesis of Peptides from α- and β-Tubulin Containing Glutamic Acid Side-Chain Linked Oligo-Glu with Defined Length. International Journal of Peptides،Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-453075
Modern Language Association (MLA)
Tegge, Werner…[et al.]. Synthesis of Peptides from α- and β-Tubulin Containing Glutamic Acid Side-Chain Linked Oligo-Glu with Defined Length. International Journal of Peptides No. 2010 (2010), pp.1-4.
https://search.emarefa.net/detail/BIM-453075
American Medical Association (AMA)
Tegge, Werner& Bonafe, Carlos F. S.& Teichmann, Aileen& Erck, Christian. Synthesis of Peptides from α- and β-Tubulin Containing Glutamic Acid Side-Chain Linked Oligo-Glu with Defined Length. International Journal of Peptides. 2010. Vol. 2010, no. 2010, pp.1-4.
https://search.emarefa.net/detail/BIM-453075
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453075