Regulation of Translational Efficiency by Disparate 5′-UTRs of PPARγ Splice Variants
Joint Authors
Shrivastava, Roopali
Medh, Jheem D.
McClelland, Shawn
Source
Issue
Vol. 2009, Issue 2009 (31 Dec. 2009), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2009-11-23
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
The PPAR-γ gene encodes for at least 7 unique transcripts due to alternative splicing of five exons in the 5′-untranslated region (UTR).
The translated region is encoded by exons 1–6, which are identical in all isoforms.
This study investigated the role of the 5′-UTR in regulating the efficiency with which the message is translated to protein.
A coupled in vitro transcription-translation assay demonstrated that PPAR-γ1, -γ2, and -γ5 are efficiently translated, whereas PPAR-γ4 and -γ7 are poorly translated.
An in vivo reporter gene assay using each 5′-UTR upstream of the firefly luciferase gene showed that the 5′-UTRs for PPAR-γ1, -γ2, and -γ4 enhanced translation, whereas the 5′-UTRs for PPAR-γ5 and -γ7 inhibited translation.
Models of RNA secondary structure, obtained by the mfold software, were used to explain the mechanism of regulation by each 5′-UTR.
In general, it was found that the translational efficiency was inversely correlated with the stability of the mRNA secondary structure, the presence of base-pairing in the consensus Kozak sequence, the number of start codons in the 5′-UTR, and the length of the 5′-UTR.
A better understanding of posttranscriptional regulation of translation will allow modulation of protein levels without altering transcription.
American Psychological Association (APA)
McClelland, Shawn& Shrivastava, Roopali& Medh, Jheem D.. 2009. Regulation of Translational Efficiency by Disparate 5′-UTRs of PPARγ Splice Variants. PPAR Research،Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-453413
Modern Language Association (MLA)
McClelland, Shawn…[et al.]. Regulation of Translational Efficiency by Disparate 5′-UTRs of PPARγ Splice Variants. PPAR Research No. 2009 (2009), pp.1-8.
https://search.emarefa.net/detail/BIM-453413
American Medical Association (AMA)
McClelland, Shawn& Shrivastava, Roopali& Medh, Jheem D.. Regulation of Translational Efficiency by Disparate 5′-UTRs of PPARγ Splice Variants. PPAR Research. 2009. Vol. 2009, no. 2009, pp.1-8.
https://search.emarefa.net/detail/BIM-453413
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453413