Hypoxia Enhances the Senescence Effect of Bortezomib—The Proteasome Inhibitor—On Human Skin Fibroblasts
Joint Authors
Cechowska-Pasko, Marzanna
Borzym-Kluczyk, Małgorzata
Krętowski, Rafał
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-01-29
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The 26S proteasome inhibitor, bortezomib, selectively induces apoptosis in some cancer cells.
However, the nature of its selectivity remains unknown.
The study presented here provides novel information on cellular effects of bortezomib in normal fibroblasts.
We have found that in normoxic conditions the percent of apoptotic cells did not change significantly, independently on incubation time and examined concentration of bortezomib (25 nmol/L or 50 nmol/L).
In hypoxic conditions we did not observe any effect of bortezomib on apoptosis of fibroblasts incubated for 24 h and 48 h in comparison to control.
Only in the case of fibroblasts incubated for 12 hours in hypoxia significant increase in apoptosis, dependent on concentration of bortezomib, was observed.
Our study has shown that bortezomib causes a time-dependent increase in senescence of normal fibroblasts, especially of these incubated in hypoxic conditions.
Moreover, we demonstrated that oxygen regulated protein 150 (ORP150) expression was induced in fibroblasts in hypoxia conditions only, suggesting that this protein may play an important role in the cytoprotective response to environmental stress.
American Psychological Association (APA)
Krętowski, Rafał& Borzym-Kluczyk, Małgorzata& Cechowska-Pasko, Marzanna. 2014. Hypoxia Enhances the Senescence Effect of Bortezomib—The Proteasome Inhibitor—On Human Skin Fibroblasts. BioMed Research International،Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-453675
Modern Language Association (MLA)
Krętowski, Rafał…[et al.]. Hypoxia Enhances the Senescence Effect of Bortezomib—The Proteasome Inhibitor—On Human Skin Fibroblasts. BioMed Research International No. 2014 (2014), pp.1-9.
https://search.emarefa.net/detail/BIM-453675
American Medical Association (AMA)
Krętowski, Rafał& Borzym-Kluczyk, Małgorzata& Cechowska-Pasko, Marzanna. Hypoxia Enhances the Senescence Effect of Bortezomib—The Proteasome Inhibitor—On Human Skin Fibroblasts. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-9.
https://search.emarefa.net/detail/BIM-453675
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-453675