Resequencing DCDC5 in the Flanking Region of an LD-SNP Derived from a Kidney-Yang Deficiency Syndrome Family
Joint Authors
Ding, Wei Jun
Duan, Azure
Wang, Mi Qu
Qin, Yu Hua
Zhang, Tian E.
Li, Wei Hong
Tan, Ling Ling
Yang, Hong Ya
Li, Wan Zhen
Zhou, Li Ping
Liu, Wei Wei
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-05-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Objective.
To explore the genetic traits of Kidney-yang deficiency syndrome (KDS).
Design.
Twelve KDS subjects and three spouses from a typical KDS family were recruited.
Their genomic DNA samples were genotyped by Affymetrix 100K single-nucleotide polymorphism (SNP) arrays.
The linkage disequilibrium (LD) SNPs were generated using GeneChip DNA analysis software (GDAS, Affymetrix).
Genes located within 100 bp of the flanks of LD SNPs were mined via GeneView.
29 exons of the doublecortin domain containing 5 (DCDC5), a representative gene within the flank of an LD SNP, were resequenced.
Results.
Five LD SNPs display midrange linkage with KDS.
Two genes with established functions, DCDC5 and Leucyl-tRNA synthetase, were mined in the flanks of LD SNPs.
Resequencing of DCDC5 revealed a nonsynonymous variation, in which 3764T/A was replaced by C/G.
Accordingly, the Ser1172 was substituted by Pro1172.
The S1172P substitution effect was evaluated as “possibly damaging” by PolyPhen.
Conclusion.
We have identified a genomic variation of DCDC5 based on the LD SNPs derived from a KDS family.
DCDC5 and other genes surrounding these SNPs display some relationships with key symptoms of KDS.
American Psychological Association (APA)
Zhou, Li Ping& Liu, Wei Wei& Zhang, Tian E.& Li, Wei Hong& Tan, Ling Ling& Li, Wan Zhen…[et al.]. 2011. Resequencing DCDC5 in the Flanking Region of an LD-SNP Derived from a Kidney-Yang Deficiency Syndrome Family. Evidence-Based Complementary and Alternative Medicine،Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-455227
Modern Language Association (MLA)
Zhou, Li Ping…[et al.]. Resequencing DCDC5 in the Flanking Region of an LD-SNP Derived from a Kidney-Yang Deficiency Syndrome Family. Evidence-Based Complementary and Alternative Medicine No. 2011 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-455227
American Medical Association (AMA)
Zhou, Li Ping& Liu, Wei Wei& Zhang, Tian E.& Li, Wei Hong& Tan, Ling Ling& Li, Wan Zhen…[et al.]. Resequencing DCDC5 in the Flanking Region of an LD-SNP Derived from a Kidney-Yang Deficiency Syndrome Family. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2011, no. 2011, pp.1-7.
https://search.emarefa.net/detail/BIM-455227
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455227