Alteration of 11β-Hydroxysteroid Dehydrogenase Type 1 and Glucocorticoid Receptor by Ethanol in Rat Liver and Mouse Hepatoma Cells
Joint Authors
Bao, Xueying
Li, Jing
Zhang, Ming
Chang, Wenguang
Wei, Shengnan
Meng, Zhao-Jie
Chen, Li
Nyomba, B. L. Grégoire
Source
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-06-01
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Alcohol is a potential risk factor of type 2 diabetes, but its underlying mechanism is unclear.
To explore this issue, Wistar rats and mouse hepatoma cells (Hepa 1–6) were exposed to ethanol, 8 g·kg−1·d−1 for 3 months and 100 mM for 48 h, respectively.
Glucose and insulin tolerance tests in vivo were performed, and protein levels of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) and glucocorticoid receptor (GR) in liver and Hepa 1–6 cells were measured.
Alterations of key enzymes of gluconeogenesis phosphoenolpyruvate carboxykinase (PEPCK) and glucose 6 phosphatase (G6Pase), as well as glycogen synthase kinase 3a (GSK3α), were also examined.
The results revealed that glucose levels were increased, and insulin sensitivity was impaired accompanied with liver injury in rats exposed to ethanol compared with controls.
The 11β-HSD1, GR, PEPCK, G6Pase, and GSK3α proteins were increased in the liver of rats treated with ethanol compared with controls.
Ethanol-exposed Hepa 1–6 cells also showed higher expression of 11β-HSD1, GR, PEPCK, G6Pase, and GSK3α proteins than control cells.
After treatment of Hepa 1–6 cells exposed to ethanol with the GR inhibitor RU486, the expression of 11β-HSD1 and GR was significantly decreased.
At the same time the increases in PEPCK, G6Pase, and GSK3α levels induced by ethanol in Hepa 1–6 cells were also attenuated by RU486.
The results indicate that ethanol causes glucose intolerance by increasing hepatic expression of 11β-HSD1 and GR, which leads to increased expression of gluconeogenic and glycogenolytic enzymes.
American Psychological Association (APA)
Meng, Zhao-Jie& Bao, Xueying& Zhang, Ming& Wei, Shengnan& Chang, Wenguang& Li, Jing…[et al.]. 2013. Alteration of 11β-Hydroxysteroid Dehydrogenase Type 1 and Glucocorticoid Receptor by Ethanol in Rat Liver and Mouse Hepatoma Cells. Journal of Diabetes Research،Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-455462
Modern Language Association (MLA)
Meng, Zhao-Jie…[et al.]. Alteration of 11β-Hydroxysteroid Dehydrogenase Type 1 and Glucocorticoid Receptor by Ethanol in Rat Liver and Mouse Hepatoma Cells. Journal of Diabetes Research No. 2013 (2013), pp.1-8.
https://search.emarefa.net/detail/BIM-455462
American Medical Association (AMA)
Meng, Zhao-Jie& Bao, Xueying& Zhang, Ming& Wei, Shengnan& Chang, Wenguang& Li, Jing…[et al.]. Alteration of 11β-Hydroxysteroid Dehydrogenase Type 1 and Glucocorticoid Receptor by Ethanol in Rat Liver and Mouse Hepatoma Cells. Journal of Diabetes Research. 2013. Vol. 2013, no. 2013, pp.1-8.
https://search.emarefa.net/detail/BIM-455462
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455462