Selection of Housekeeping Genes for Transgene Expression Analysis in Eucommia ulmoides Oliver Using Real-Time RT-PCR
Joint Authors
Gyokusen, Mayumi
Chen, Ren
Nakazawa, Yoshihisa
Gyokusen, Koichiro
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-03-17
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In order to select appropriate housekeeping genes for accurate calibration of experimental variations in real-time (RT-) PCR results in transgene expression analysis, particularly with respect to the influence of transgene on stability of endogenous housekeeping gene expression in transgenic plants, we outline a reliable strategy to identify the optimal housekeeping genes from a set of candidates by combining statistical analyses of their (RT-) PCR amplification efficiency, gene expression stability, and transgene influences.
We used the strategy to select two genes, ACTα and EF1α, from 10 candidate housekeeping genes, as the optimal housekeeping genes to evaluate transgenic Eucommia ulmoides Oliver root lines overexpressing IPPI or FPPS1 genes, which are involved in isoprenoid biosynthesis.
American Psychological Association (APA)
Chen, Ren& Gyokusen, Mayumi& Nakazawa, Yoshihisa& Gyokusen, Koichiro. 2010. Selection of Housekeeping Genes for Transgene Expression Analysis in Eucommia ulmoides Oliver Using Real-Time RT-PCR. Journal of Botany،Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-455753
Modern Language Association (MLA)
Chen, Ren…[et al.]. Selection of Housekeeping Genes for Transgene Expression Analysis in Eucommia ulmoides Oliver Using Real-Time RT-PCR. Journal of Botany No. 2010 (2010), pp.1-7.
https://search.emarefa.net/detail/BIM-455753
American Medical Association (AMA)
Chen, Ren& Gyokusen, Mayumi& Nakazawa, Yoshihisa& Gyokusen, Koichiro. Selection of Housekeeping Genes for Transgene Expression Analysis in Eucommia ulmoides Oliver Using Real-Time RT-PCR. Journal of Botany. 2010. Vol. 2010, no. 2010, pp.1-7.
https://search.emarefa.net/detail/BIM-455753
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-455753